Spirulina platensis is a blue-green alga with columnar cells forming a spiral-like twisted (helix). This research aimed to boost cell growth and the simultaneous production of high-value compounds in response to the varying concentration of MSG as metabolic stressors in Spirulina platensis. The methods used in this study included biomass growth as measured by the cell density method with a wavelength of 680nm, while phycocyanin concentration and phycocyanin purity were measured by wavelengths of 620 and 280nm. Carotenoid content was measured using a spectrophotometer, while total lipids were extracted by Bligh and Dyer method, and then fatty acids profiling was evaluated using GC-MS. In the total lipid data, a drastic increase occurred at a concentration of 7.5 mM which was about 3 times higher than the control and 5 mM MSG. The results showed that the addition of monosodium glutamate had a significant effect on the biomass growth rate of microalgae, but it could increase the phycocyanin content.  The greatest value of phycocyanin concentration was obtained with the addition of 7.5 mM monosodium glutamate which was 0.116 mg/mL, compared to the control which was 0.060 mg/mL. While the purity at the best concentration of 7.5 mM monosodium glutamate was 2.049 ± 0.159 and 1.518 ± 0.266 for the control. In this study, it was shown that sodium glutamate is a good metabolic stress in the growth of phycocyanin production from the microalgae Spirulina platensis.