Zooplankton communities possess a major position in freshwater ecosystems, which are essential in maintaining a biological balance of freshwater habitats. Knowledge about processes and mechanisms for affecting variations in abundance, biomass and diversity of zooplankton is important for maintaining a biological balance of freshwater ecosystems. Rotifera plays an important role in different aquatic food webs. The main objective of this study was to identify the most abundant types of rotifers at sampling stations of El-Mahmoudia Canal. The three sampling stations detected one class of rotifers, including four different orders. These orders were represented by 20 families, including 92 rotifer species with an annual average of 36833 organisms/ m3. The maximum average counts were recorded during autumn (49000 organisms/m3), while the minimum average was recorded during spring (24500 organisms/m3). The highest rotifer average was recorded at El-Mahmoudia City (station III) (37000 organisms/m3); encounter Damanhour City (station II) characteristics with average counts of rotifers (36625 organisms/m3). Data in the present study illustrated that transparency (0.23-0.42 m), temperature (19-31.1 oC), depth (1.7-2.3 m), salinity (0.1-0.9 ‰), water pH (7.1-8.6) and dissolved oxygen (2.24-7.20 mg/l), which influence the Rotifera distribution and abundance.