Biological tracking refers to the use of microorganisms' physical reactions to evaluate environmental changes. The relationship between algal, protozoan and related physical-chemical properties in the highly industrial Helwan and El-Tebbin area was the focus of this study. Four sites are included in the study region south of Helwan City: El-Shorafa (I, II), El-Hekr (Canals) and El-Tebbin drain. An excellent indication of the chemical quality of surface waters is the analysis of chemical parameters for water. The concerns of water chemistry and ecological condition are integrated into biological metrics, which are used as a guide for evaluating biological communities. Various environmental factors as well as the populations of algae and protozoa were addressed. Certain algae species and other factors were found to be correlated using statistical analysis and Palmer's index of pollution. The final findings showed that the El-Tebbin industrialized region is a somewhat organically polluted area due to the presence of major microalgae such as Synedra, Chlorella, Cyclotella, Melosira and Pediastrum that are thought of as bio-indicators. Furthermore, the presence of species of ciliated protozoa that are thought to be the most pollution-tolerant include Vorticella, Tintinnidium, Strombidiom, Burselopsis and Arcella. Utilizing living organisms to bio-monitor organic and inorganic contaminants has recently attracted increasing interest. This study suggests using the recorded bio-indicators in organic pollution management.