The growth parameters of the freshwater puffer fish Tetraodon lineatus from Lake Nasser, Egypt were estimated for the first time. A total of 2691 samples were monthly collected from commercial fishing boats at landing sites along the lake from October 2017 to September 2018. The total length of the samples ranged from 13.8 to 50.0cm, with an average of 24.71cm, and the total weight ranged from 63.7 to 2827.1g, with an average of 374.65 g. The length-weight relationship revealed a negative allometric growth for males, females, and combined sexes, with an insignificant difference between males and females at (P> 0. 05). The maximum monthly condition factor was recorded in March for males (2.13) and in January for both females (2.07) and combined sexes (2.22). The age of 533 samples was determined by using annual rings of their vertebrae. The results showed that three samples had the highest age (8 years), while 16 samples had the smallest age (0 years). The estimated asymptotic length of the investigated species was 57.2 cm; the growth coefficient (instantaneous rate) (K) was 0.143 year^-1; t_o was -1.89 year; the growth performance index (∅) was 2.67, and longevity (t_max) was 12.02 years. The results of this study are among the first of their kind in the biological data on the freshwater pufferfish in Lake Nasser. The findings of the current study add more insight into the T. lineatus age and growth model which would help in establishing management plans for Lake Nasser fisheries.