Ismailia Canal is considered one of the most important sources of fresh water for a large number of citizens, yet it is exposed to many sources of pollution. Water and fish samples were collected to study the quality of water as well as the concentration of metals in water, fish tissue, and its effect on humans. Several indices were used to evaluate the water and fish quality of Ismailia Canal water.  Based on the weighted arithmetic water quality index (WAWQI) results, the canal water was classified as excellent for drinking, aquatic life and irrigation. While the study of Metal Index (MI) showed that the water of the Ismailia Canal is polluted for drinking water and aquatic life while it is not polluted for Irrigation water, as well as the resultant contamination index (Cd) values ranged between low (Cd < 1) to high (Cd > 3) in the station ( 1) due to the effluent of drainage water treatment plants which throwing waste water rich with Aluminum, Iron and Manganese. Aquatic Toxicity Index (ATI) results indicate the suitability of the canal's water for different fish species. Hazard Index (HI) and Hazard Quotient (HQ) indicate that no risk (HQ < 1) on the other hand (HI) is 1.538. This result revealed the moderate risks from the consumption of edible tissue of the Nile Tilapia (O. niloticus). For improvement of water quality in Ismailia canal, the dumping of pollutants of all kinds, industrial, agricultural and domestic, into the waters of the Ismailia Canal must be prohibited and criminalized.