This study was conducted at Sids Horticultural Research Farm. Beni-Suef Governorate in Egypt in the 2021, 2022, and 2023 seasons to assess the effectiveness of eight treatments on Flame seedless grapevine productivity and weed control. At harvest, chemical herbicidal residues in the berries were monitored using high-performance liquid chromatography (HPLC) in a randomized complete block design with four replicates. The findings indicate that Roundup should be applied at 2.5 L/fed, Starane at 0.2 L/fed, black polyethylene mulch, and rice straw mulch. Additionally, Egyptian clover treatments provided 88.1–97.4% weed control. Furthermore, these treatments greatly enhanced grapevine growth and fruiting. The amount of herbicide residues was less than the maximum permitted limit. An analysis of the economics revealed that, growing grapevines with Egyptian clover, black plastic mulch, or rice straw mulch is a viable endeavor for producers. Mulching and Egyptian clover are both excellent, non-toxic options for controlling weeds in vineyards.