To investigate the impact of VitE, human chorionic gonadotropin (hCG), and VitE+hCG on milk yield and blood cortisol concentrations in pregnancy and non-pregnancy under cold months. Thirty-four Holstein healthy milking cows were assigned randomly into four groups (control, VitE alone, hCG alone, and VitE+hCG). These groups were injected IM with 1.0 ml propylene glycol/animal, 1.39 ml α-tocopherol/animal three times before mating for ten days, 2000 IU hCG/animal once post-mating for one week, and 1.39 ml+ 2000 IU/animal as the same doses and dates as in VitE and hCG groups, respectively. Results revealed that treatments did not influence cortisol concentrations during mating in pregnancy and non-pregnancy. The combination group affected significantly the cortisol than other treatments in pregnant and (P> 0.05) in non-pregnant cows. The total cortisol concentrations decreased (P< 0.05) in the combination of pregnant and non-pregnant cows. Temperature-Humidity index (THI) was ≥ 46. Milk yield increased significantly in VitE and combination groups during mating and pregnancy phases (not non-pregnancy). The total milk yield was significantly greater in VitE and VitE+HCG of pregnant cows in non-pregnant cows. Conclusively, the cold weather didn't affect cortisol concentrations in mating, pregnancy, and non-pregnancy, and the combination group resulted in low cortisol in pregnancy, probably due to the THI comfortable index ≥ 46 or VitE as a potential antioxidant, leading to high milk yield and low cortisol in pregnancy and non-pregnancy. In conclusion, the treatments by VitE and combination (VitE+hCG) enhanced the milk yield and reduced the cortisol level under the cold weather.