Honeybees exposed to pesticides direct or indirect ways through their food research trips.  Herein, this study examined the toxicity effects on laboratory of seven tested pesticides insecticides from different pesticide groups. emamectin benzoate, imidacloprid, chlorpyrifos, indoxacarb, lambada cyhalothrine, glyphosate and thiophanate-methyl on honeybee workers, Apis mellifera L. through different exposure periods of time at 24, 48, and 72 hours. The selected pesticides were ranked based on their toxicity from the most to least one as following: emamectin benzoate, imidacloprid, chlorpyrifos, indoxacarb, and lambda cyhalothrine. Further, thiophanate-methyl and glyphosate were the lowest toxicity among selected pesticides. The highest toxic pesticide during the exposure period 24, 48 and 72 hrs was emamectin benzoate with LC₅₀ values (0.247, 0.047 and 0.020 ppm) and with LC₉₀ values (5.752, 0.302 and 0.072 ppm) respectively, and the least one was glyphosate with LC₅₀ values of (6861.151, 3366.968 and 2477.267 ppm) and LC₉₀ values of (28243.795, 9033.695 and 6203.485 ppm) after 24, 48 and 72hr of exposure respectively. However, chlorpyrifos ranked the third toxic pesticide at 24 and 48 hrs of treatment with LC₅₀ values of 10.226 and 2.731 ppm and with LC₉₀ values of (101.224 and 7.496 ppm for 24 and 48 hrs, respectively). Whereas, after 72 hrs chlorpyrifos recorded the fourth toxic pesticide with LC₅₀2.086 ppm and LC₉₀ 5.179ppm. Based on the toxicity index, results showed that, the most to least toxic pesticides were arranged as followed: emamectin benzoate>imidacloprid >chlorpyrifos >indoxacarb >lambada cyhalothrine> thiophanate -methyl > glyphosate. These results demonstrated that pesticides are very toxic to honeybee workers and must avoid applying them during the times when bees are most active such as during flowering periods.