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395086

Mosquitocidal Activity of Extracts Derived from Soil Actinomycete Isolates..

Article

Last updated: 23 Dec 2024

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Subjects

-

Tags

Plant pest management

Abstract

This study investigates the insecticidal activity of 15 extracts of actinomycete isolates (NAM-1-NAM-15) sourced from diverse soil samples because the actinomycete strains were isolated from soil only against the third instar larvae of Culex pipiens. The isolates were cultured on starch casein agar, purified, subjected to fermentation add then solvent extraction to obtain the crude extracts. In primary Screening of the ethyl acetate extracts at a concentration of 1.0 mg/ml, NAM1, NAM2, and NAM4 achieved 100% mortality within one hour, indicating a high acute toxicity of these three extracts, while the rest of isolates exhibited a minimal toxic effect, with mortality rates as low as 3.33%. After six hours, NAM15 and NAM13 demonstrated moderate toxicity, with mortality rates of 46.67% and 36.67%, respectively. After 24 hours, all extracts (except NAM3) reached 100% mortality, indicating their insecticidal properties. LC50 values showed that NAM1 had the highest toxicity at 0.21 mg/ml, followed by NAM4 and NAM2 at 0.52 mg/ml and 0.60 mg/ml, respectively.Biochemical analyses revealed distinct characteristics among isolates, with NAM2 exhibiting the highest enzyme activity, particularly on lipase and protease. This research confirms the potential of local actinomycete strains as effective biological agents for sustainable mosquito control, contributing to environmentally friendly pest management strategies.

DOI

10.21608/asejaiqjsae.2024.395086

Keywords

Actinomycetes Isolation, Culex pipiens, larvicidal activity, biological control, LC50 values, hydrolytic enzymes

Authors

First Name

Nagwa

Last Name

Al-Nagar

MiddleName

M.A.

Affiliation

plant protection research institute agricultural research center, sabahia , alexandria, egy

Email

nagwaelnagar17@yahoo.com

City

Alexandria

Orcid

-

First Name

Mohamed

Last Name

S. Shawir

MiddleName

-

Affiliation

Department of Pesticide Chemistry and Technology, Faculty of Agriculture, Alexandria University, El-Shatby, Alexandria, Egypt.

Email

-

City

-

Orcid

-

First Name

Samir

Last Name

A. M. Abdelgaleil

MiddleName

-

Affiliation

Department of Pesticide Chemistry and Technology, Faculty of Agriculture, Alexandria University, El-Shatby, Alexandria, Egypt.

Email

-

City

-

Orcid

-

First Name

Hamdy

Last Name

K. Abou Taleb

MiddleName

-

Affiliation

Plant Protection Research Institute, Agricultural Research Center, Sabahia, Alexandria, Egypt.

Email

-

City

-

Orcid

-

First Name

Ahmed

Last Name

I. R. Khalil

MiddleName

-

Affiliation

Institute of Graduate Studies and Research, Alexandria University

Email

-

City

-

Orcid

-

Volume

45

Article Issue

4

Related Issue

51107

Issue Date

2024-12-01

Receive Date

2024-10-25

Publish Date

2024-12-30

Page Start

719

Page End

729

Print ISSN

1110-0176

Online ISSN

2536-9784

Article File

ASEJAIQJSAE_Volume 45_Issue 4_Pages 719-729.pdf

PDF . 283.7kB

Link

https://asejaiqjsae.journals.ekb.eg/article_395086.html

Detail API

https://asejaiqjsae.journals.ekb.eg/service?article_code=395086

Order

13

Type

Original Article

Type Code

53

Publication Type

Journal

Publication Title

Alexandria Science Exchange Journal

Publication Link

https://asejaiqjsae.journals.ekb.eg/

MainTitle

Mosquitocidal Activity of Extracts Derived from Soil Actinomycete Isolates..

Details

Type

Article

Created At

23 Dec 2024