Beta
ManageSearchChatDiscover
365960

Polymorphism Detection of some Transcription Factors Genes Conferring Drought Tolerance in Diverse Wheat (Triticum aestivum L.) Genotypes

Article

Last updated: 01 Jan 2025

OverviewSimilar Items

Subjects

-

Tags

Crop genetics and breeding

Abstract

Drought is considered the most hazardous environmental abiotic stress factors, which adversely affects wheat production (Triticum aestivum L.). Several transcription factor (TF) families, such as WRKY, MYB, and NAC, have an important role in response to drought stress in plants. The investigation of NaC and WRKY genes is considered a new area for research, especially in Egypt. Thus, this study aimed to detect the three genes namely, TaNAC2a, TaWRKY2, and TaWRKY19 by using DNA marker based on encoding regions in different wheat genotypes. In addition to that, to identify the polymorphism in TaNAC2a gene by performing sequence analysis of the central part of exon 2 in seven wheat cultivars. The TaNaC2a, TaWRKY19, and TaWRKY2 genes were detected in most of the tested wheat genotypes. The polymorphic bands detected for TaNAC2a, TaWRKY19, and TaWRKY2 suggest the existence of other alleles at the same locus of these genes. The central part of exon2 was sequenced (260 bp) showing the conserved sequence in all cultivars, except the positions from 813 to 863 were high polymorphic. Detected SNPs related to drought tolerance were not found. The amino acids sequence encoded from the target DNA region was highly conserved from position 282 to 319 in the N-terminal domain in all genotypes except a few positions. Detection of these important genes is considered a critical start point for further study especially gene expression of these genes.

DOI

10.21608/asejaiqjsae.2024.365960

Keywords

Drought tolerance, NAC genes, Polymorphism, WRKY genes, Wheat

Authors

First Name

Huda

Last Name

Shakam

MiddleName

Mohammmed

Affiliation

Genetics department,Faculty of Agriculture (EL-Shatby),Alexandria University,Alexandria,Egypt.

Email

hoda.ibrahim@alexu.edu.eg

City

الاسكندرية

Orcid

0009-0002-2043-9929

First Name

Mohamed

Last Name

Ebaid

MiddleName

-

Affiliation

Plant Production Department, Arid Lands Cultivation Research Institute (ALCRI), City of Scientific Research and Technological Applications (SRTA-City), New Borg El-Arab City, Alexandria 21934, Egypt.

Email

mohamedebaid979@gmail.com

City

Alexandria

Orcid

-

First Name

Doaa

Last Name

Hamza

MiddleName

A

Affiliation

Wheat Research Department, Field Crop Research Institute, ARC, Egypt.

Email

doaahamza1@yahoo.com

City

Alexandria

Orcid

-

Volume

45

Article Issue

2

Related Issue

47194

Issue Date

2024-06-01

Receive Date

2024-05-25

Publish Date

2024-06-30

Page Start

307

Page End

319

Print ISSN

1110-0176

Online ISSN

2536-9784

Article File

ASEJAIQJSAE_Volume 45_Issue 2_Pages 307-319.pdf

PDF . 590.9kB

Link

https://asejaiqjsae.journals.ekb.eg/article_365960.html

Detail API

https://asejaiqjsae.journals.ekb.eg/service?article_code=365960

Order

8

Type

Original Article

Type Code

53

Publication Type

Journal

Publication Title

Alexandria Science Exchange Journal

Publication Link

https://asejaiqjsae.journals.ekb.eg/

MainTitle

Polymorphism Detection of some Transcription Factors Genes Conferring Drought Tolerance in Diverse Wheat (Triticum aestivum L.) Genotypes

Details

Type

Article

Created At

23 Dec 2024