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285162

The Humoral Immunological Response and Bio-Efficiency Evaluation to Entomopathogenic Nematodes as A Biopesticide on Spodoptera littoralis (Boisd)

Article

Last updated: 23 Dec 2024

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Subjects

-

Tags

Economic entomology

Abstract

The current study aims to investigate the residual toxicity and nutritional indices of entomopathogenic nematodes (EPNs), as well as immunological response to an infection in Spodoptera littoralis. Compared to the individual impacts of Steinernema carpocapsae, Heterorhabditis indica and EPNs binary combinations showed a higher pathogenicity to S. littoralis according to mortality, physiological responses, and humoral immune. The anti-feedant activity percentages of H. indica and S. carpocapsae treated larvae had a more potent effect than their binary mixture at LC35, compared to full-feeding activity in the control. The biochemical characteristics of infected hemolymph showed a significant variation, not only in the total protein concentration, but also in the appearance of the new protein bands profile. An additional band, with a molecular weight of ~79 KDa was observed at immunized hemolymph, it has been postulated that it may be the phenoloxidase(PO), which is in close agreement with the significant induction of PO activity after infection. Interestingly, the immunised hemolymph of the larvae infected with binary combinations of EPNs showed a notably increased intensity of the major proteins and a rise in the number of antimicrobial peptides with molecular weights under 30 KDa. However, the significant reduction of total protein count and PO level, which ultimately resulting in larval death, is evidence that the binary mixture of the investigated nematodes is highly suppressed S. Littoralis larval immune system.
 

DOI

10.21608/asejaiqjsae.2023.285162

Keywords

Entomopathogenic nematodes, humoral immunological response, bio-efficiency evaluation, Spodoptera littoralis (boisd)

Authors

First Name

Rima

Last Name

Shahin

MiddleName

-

Affiliation

Department of Applied Entomology and Zoology, Faculty of agriculture (El-Shatby), Alexandria University, Alexandria 21545, Egypt

Email

rimashahin123@yahoo.com

City

-

Orcid

0000-0002-8473-3847

First Name

Wael

Last Name

Khamis

MiddleName

M

Affiliation

Plant Protection Research Institute, Agricultural Research Center, Al-Sabhia, Alexandria, Egypt

Email

waelmkhamis2019@yahoo.com

City

-

Orcid

0000-0003-1139-7907

First Name

Ahmed M.

Last Name

El-Sabrout

MiddleName

-

Affiliation

Applied Entomology and Zoology Department, Faculty of Agriculture(El-Shatby), Alexandria University. Egypt.

Email

elsabroutahmed@alexu.edu.eg

City

-

Orcid

0000-0003-1139-7907

Volume

44

Article Issue

1

Related Issue

39130

Issue Date

2023-03-01

Receive Date

2023-01-15

Publish Date

2023-03-31

Page Start

15

Page End

24

Print ISSN

1110-0176

Online ISSN

2536-9784

Article File

ASEJAIQJSAE_Volume 44_Issue 1_Pages 15-24.pdf

PDF . 473.1kB

Link

https://asejaiqjsae.journals.ekb.eg/article_285162.html

Detail API

https://asejaiqjsae.journals.ekb.eg/service?article_code=285162

Order

2

Type

Original Article

Type Code

53

Publication Type

Journal

Publication Title

Alexandria Science Exchange Journal

Publication Link

https://asejaiqjsae.journals.ekb.eg/

MainTitle

The Humoral Immunological Response and Bio-Efficiency Evaluation to Entomopathogenic Nematodes as A Biopesticide on Spodoptera littoralis (Boisd)

Details

Type

Article

Created At

23 Dec 2024