The present study was conducted to evaluate the environmental hazards of insecticides used as seed dressing by Gausho and leaf spraying by Tiliton on abundance and diversity of mites inhabiting soil in which they are useful in nutrient cycling in soil. Soil samples were collected monthly from cotton field during the vegetation period (April-September). Gausho caused a complete mortal effect on soil mite individuals over one month after the insecticide application. The mortal effect is suggested due to the fast leaching of insecticide into the soil by dipping technique method. Soil mites' population was maximum in June. Foliar spraying of Tiliton reduced the population density of soil mites compared with the control till the end of vegetative period. A reduction of 57.65% of soil mites was caused due to insecticides usage. Mortality may relate to the incidence dropping off some pesticides droplets into the soil during spraying method. Gausho reduced the species diversity (H'≤ 3) of oribatid mites 60 days after seed soaking, while exhibited  a very  low diversity (H'≤ 1) for prostigmatid, mesostigmatid, and astigmatid mites. Thus, the insecticide application in conventional fields had a significant effect on non-target beneficial arthropods and therefore reduced biological aspects in soil ecosystem that considered an indicator of soil quality and ensures its sustainability.