Sandy soils are well known with its low fertility (i.e., soil nutrient deficiency stress), which mainly depends on the level of soil organic matter. Sandy soils globally cover around 900 million ha, which particularly spread in arid and semi-arid regions. The cultivation of such soils is a great challenge worldwide specially to produce biofortified different crops. The current study was designated to investigate the differences between pepper and green bean to different forms and applied doses of selenium during biofortification program under soil nutrient deficiency stress. The main forms of selenium were biological nano selenium and its bulk form, whereas the main doses were 10, 20, 30, and 40 ppm for both forms. In general, the response of studied vegetable crops had a similar behavior under such studied stress regarding all selected attributes. This response represents in increasing the studied trails of growth, and nutritional status of both crops by increasing the applied nano-Se up to 20 or 30 ppm, while bulk forms of Se at 20 ppm for both crops. The production of biofortified fruits of both green bean and pepper were not only contain high content of Se to prevent the biofortification program, but also increased the all studied attributes of crops. This study seeks to remedy the problems of cultivation of sandy soils under biofortification program by applying both mineral and nano-Se sources with propriety to the biological nano-Se. This study also opened many questions concerning the biofortification program using other vegetable crops under different stresses.