The house fly, M. domestica L., is a nuisance insect pest that transmits many pathogens. In this study, four pesticides belong to four different groups “Neonicotinoid (Imidacloprid /Kita Pride® 35% SC), Pyrethroid (Beta-cyfluthrin /Blendo spicial® 10% EW), Organophosphate (Primiphos-methyl /Primi one® 50% EC) and Phenylpyrazole (Fipronil /EG-Fipro® 5% SC)" were evaluated for controlling the house fly. Toxicologically, the result showed that the LC50 of fipronil is 0.0064 mg/kg, followed by Imidacloprid 0.0033 mg/kg, then Beta cyfluthrin and primiphos-methyl 0.0002 and 0.00017mg/kg, respectively. Bio-chemically, the insecticides used affected the total protein content and the highest effect was for Beta-cyfluthrin (54.53±1.34, 41.33±1.26mg/g.b.wt) in adult and larvae, respectively. It also affected the activity of some enzymes (Acetylcholinesterase, Glutathione S-transferase and Non-specific esterase) compared to the control. Therefore, Primiphos-methyl was the most toxic of the other insecticide formulations in the case of adult insects, while fipronil was the most toxic in the case of larvae. And the highest effects on enzyme activity were for beta-cyfluthrin and fipronil, both in larvae and adults.