The biochemical and histopathological changes of abamectin. sewage and growth hormones to the Nile tilapia fish O.niloticus L. 60gm bodyweight were studded. The obtained data show that exposed fish to sublethal concentrations of abamectin, sewage (50% diluted with clean water) and growth hormone (6 mg/kg) for21 days caused some biochemical histopathological changes in the Nile tilapia fish. O.niloticus L. Sewage caused an Increase in the level of all serum blood components that took place during the experimental period. growth hormone caused an increase in the level of ALT, AST, glucose, total protein, albumin, uric acid, creatinine and cholesterol took place during the experimental period. On the other hand, the histopathology studies show degeneration of hepatocytes, and congestion of blood vessels and revealed degenerative changes and cystic dilatation of some renal tubules together with congestion of renal blood vessels and mild to moderate lymphocytic infiltration were detected compared with the control group.