A pot experiment was carried out in the greenhouse of the National Research Center, Dokki, Cairo, Egypt during summer season of 2018 to study the role of proline and glutamic acid on dry matter, photosynthetic pigments and mineral status of cowpea plants. Generally, application of amino acid (glutamic and proline) improved growth traits stem and top dry weight resulted from glutamic acid treatment exceeded those obtained from proline spraying. The concentration of nitrogen increased by both treatment of amino acids but the increase by proline exceeded those by glutamic acid. Phosphorus increased only by proline treatment while potassium concentration rose approximately with similar trends either by proline or glutamic acid). A positive relationship was found between the concentration of N and spraying with amino acids. Under regular irrigation or drought at fruiting proline induced the high effect than glutamic acid or control treatment while under drought at flowering proline or glutamic acid induced approximately the same increase. Concerning K concentration in seeds of cowpea, it showed approximately the same response of P concentration with both amino acids exogenous spraying under moisture stress at flowering or fruiting stages as well as with regular irrigation all over the growth stages of cowpea plants.