Liver transplantation (LT) has developed as a well-established therapeutic option for end-stage liver disease, specific primary and a few secondary hepatic malignancies, and a few uncommon metabolic liver disorders. The rate of rejection in the transplant population has significantly decreased because the use of immunosuppressive medications, immunosuppressive has increased patient sensitivity to opportunistic infections. For patients with LT, infections continue to be a significant source of morbidity and mortality. 180 urine and stool samples from 30 LT patients at Mansoura University's Gastroenterology Surgical Center (GEC), Egypt, were included in this investigation from March 2021 to March 2023. Fourteen commercial antibiotics belonging to different families with different modes of action were used. 16S rRNA was done to confirm the identification of Klebsiella pneumoniae (K. pneumoniae) by detecting the 16S rRNA gene. This study's findings demonstrated that K. pneumoniae strains are entirely resistant to seven different kinds of antibiotics Nitrofurantoin, Ceftazidime, Amoxicillin, Vancomycin, Tobramycin, Erythromycin, and Gentamicin, and the most effective type of antibiotic was Amikacin (50%). This study focuses on the surveillance of K. pneumoniae in liver transplant patients before and after the surgery. It also demonstrates the resistance levels of the isolated K. pneumoniae against different antibiotics. Among various antibiotics used, Amikacin remains the most effective antibiotic (with 50% effectiveness). Therefore, this study underscores Amikacin's potential role in managing infections in LT patients. These results are crucial for developing targeted therapeutic strategies and improving patient outcomes in liver transplantation.