Background: Oxygen is one of the most necessary components of life, so the nurses must be familiar with its administration and its potential hazards. Oxygen administration is useful when it is performed with the proper amount, but incorrect usage and inadequate practices of oxygen administration may lead to complications associated with increased morbidity and mortality. Aim of the study: This study aimed to assess quality of nursing performance regarding oxygen administration for high risk infant. Design: A descriptive cross sectional study was utilized for the current study. Setting: This study was conducted at the neonatal intensive care unit and pediatric intensive care unit at Beni-Suef University Hospital affiliated to Ministry of Higher Education. Sample: A purposive sample of 80 nurses working in the previously mentioned setting was included in the study. Tools: Three tools. The first tool was a structured interviewing questionnaire to assess nurses' knowledge regarding oxygen administration; the second tool was an observational check list to assess nurses practice regarding oxygen administration and the third tool was attitude scale to assess nurses' attitude toward oxygen administration. Results: The study findings revealed that 53.75% of the studied nurses had a good level of total knowledge regarding oxygen administration for high risk infant, while 65% of the studied nurses had a competent level of total practice regarding oxygen administration for high risk infant and 66.25% of the studied nurses had a positive attitude regarding oxygen administration for high risk infant. Conclusion: it can be concluded that, the majority of the studied nurses had a good level of knowledge, a competent level of practice, and a positive attitude regarding oxygen administration. Recommendation: The study recommended that upgrading nurses' performance through the continuous application of an education program for nurses about oxygen administration.