Multiseptate gallbladder is a rare congenital anomaly that may be silent or with symptoms. Herein, we report a case of 12-year-old male child with abdominal pain associated with nausea and vomiting. The diagnosis of multiseptate gallbladder was settled at 6 months after the first episode of abdominal pain by screening ultrasound and was confirmed by magnetic resonance cholangiopancreatography. We performed laparoscopic cholecystectomy with complete resolution of preoperative symptoms. The patient was discharged on postoperative day 1. The postoperative course was uneventful without complications.