In recent times, considerable research endeavours have been directed towards
reducing the magnesium content in phosphate rock concentrate, making it a viable
raw material for phosphoric acid production and allied industries. One noteworthy
approach in this regard is the utilization of bioprocessing techniques. Nonetheless,
this investigation is centered on the utilization of an enzyme obtained from
Aspergillus Niger fungi cultivated on coffee waste sourced from the National
Research Center Microbiology Laboratory. This enzyme is employed as a surface
modifier during fatty acid flotation for calcareous phosphate rock. A blend of oleic
acid and kerosene serves as the collector. A comprehensive exploration of various
factors impacting enzyme activity, such as enzyme dosage, solution pH,
temperature, and conditioning time, has been undertaken, aligning with the
demands of local, regional, and global markets. Under optimal conditions, the
study achieved a concentrate product containing less than 0.8% MgO from the
Abu-Tartur feed, originally containing 2.88% MgO, with a remarkable P2O5
recovery exceeding 88%.