The most prevalent and serious illness in the world, otitis media, is typified by
fluid accumulation in the middle ear along with acute infection signs and symptoms,
which are brought on by bacterial infections. Due to increasing of multi drug resistance
bacteria we are in this study use marine Streptomyces specially its secondary metabolites
against these multi drug resistance bacteria after isolation and prove that they affected
these bacteria. Forty-four are actinomycetes isolates were taken from marine habitats
including Hurgada marine sea, Ras Sedr sediment, Ain Sokhna sediment marine sea
water, while thirty-six bacterial isolates were obtained from Kafr El-Sheikh General
Hospital, Benha University Hospital and Mabaret El-Asafra hospital. The antimicrobial
activities of ethyl acetate extracts for isolated Streptomyces spp. Tested using the agar disc
diffusion method. 50 µl of each extract culture were soaked on filter paper discs and
placed on neutral agar media seeded with isolated bacteria. Antimicrobial activity for
Streptomyces extracts isolated from three regions is observed against isolated bacteria