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372264

Microbial Interdisciplinary Experiment: Unravelling the outcome of Lglutaminase Produced by Streptomycetes isolates

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Last updated: 29 Dec 2024

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Abstract

It is well known that Streptomyces produces beneficial pharmaceuticals, particularly antibiotics
and anticancer agents. This investigation uncovered 34 randomly selected streptomycetes isolates
in sediment, marine water, soil, and other habitats. Using a qualitative rapid plate assay to test for
GluNase synthesis, 9 of them produced a significant amount of L-glutamine pink colour.The
maximum levels of GluNase synthesis were obtained at a pH of 7.5, an incubation temperature
of 40 °C, and the utilization of glucose and peptone as the carbon and nitrogen sources,
respectively. 

DOI

10.21608/jbes.2023.372264

Keywords

Streptomycetes, GluNase, isolation, Screening, optimiza

Authors

First Name

Mohamed O.

Last Name

Abdelmonem

MiddleName

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Affiliation

Botany and Microbiology Department, Faculty of Science, Benha University, Benha, Egypt.

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Orcid

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First Name

Mohamed E.

Last Name

El awady

MiddleName

-

Affiliation

Microbial Biotechnology Department, Biotechnology Research Institute, National Research Centre, El-Buhouth St. 33, Dokki, Cairo, Egypt.

Email

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City

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Orcid

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First Name

Mohamed I.

Last Name

El-khonezy

MiddleName

-

Affiliation

Molecular Biology Department, National Research Centre, 33-El Bohouth St., Dokki, Giza, P.O.12622, Egypt.

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City

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Orcid

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First Name

Mervat G.

Last Name

Hassan

MiddleName

-

Affiliation

Botany and Microbiology Department, Faculty of Science, Benha University, Benha, Egypt.

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City

-

Orcid

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First Name

Mona A.

Last Name

Kishk

MiddleName

-

Affiliation

Botany and Microbiology Department, Faculty of Science, Benha University, Benha, Egypt.

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Orcid

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Volume

10

Article Issue

4

Related Issue

49687

Issue Date

2023-10-01

Receive Date

2024-08-06

Publish Date

2023-10-01

Page Start

114

Page End

129

Print ISSN

2536-9202

Online ISSN

2356-6388

Article File

JBES_Volume 10_Issue 4_Pages 114-129.pdf

PDF . 381.7kB

Link

https://jbes.journals.ekb.eg/article_372264.html

Detail API

https://jbes.journals.ekb.eg/service?article_code=372264

Order

372,264

Type

Original Article

Type Code

3,063

Publication Type

Journal

Publication Title

Journal of Basic and Environmental Sciences

Publication Link

https://jbes.journals.ekb.eg/

MainTitle

Microbial Interdisciplinary Experiment: Unravelling the outcome of Lglutaminase Produced by Streptomycetes isolates

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Type

Article

Created At

21 Dec 2024