Hepatitis C virus (HCV) infection is complicated by microbial translocation throughout the movementofliving bacteria or their endotoxins from the intestinal lumen to the mesenteric lymph nodes andotherextraintestinal locations. The present study aimed to evaluate bacterial translocation in patients withHCVinfection pre and post-treatment. Results showed that HCV titer was high in pretreated patients andlowinpost-treated patients, the isolated bacteria were identified according to morphological cultures, theVITEK-2-system, and antibiotics. It was found that 76% of pretreated patients gave bacterial isolates anddividedthem according to symptoms; 16% showed mild symptoms, 49.33% showed moderate symptomsand10.66% showed severe symptoms. The isolated bacteria were mostly multi-drug resistant. Thefrequencypercent of trans-located bacteria was 29.33% for Gram-positive cocci bacteria included(25.33%Streptococcus spp and 4% Staphylococcus spp), and 46.66% for Gram-negative bacilli bacteriaincluded(33.33% E.Coli and 13.33% Klebsiella spp). 6.66% of post-treated patients gave bacterial growthas2.66%showed moderate symptoms, and 4% showed severe symptoms. The percent of bacteria was 5.33%forGram-positive cocci bacteria included (4% Staphylococcus spp and 1.33% Streptococcus spp), and1.33%for Gram-negative bacilli bacteria. According to the studied patients, Results showed a significant differencebetween pre and post-treated patients as the highest percentage of bacterial growth was foundinthepretreated patients and more bacterial isolates were from patients with severe symptoms while most samplesthat gave no growth were from patients who showed mild symptoms.