Artemisia herba-alba, is a profoundly significant medicinal plant, known for the abundant spectrum of bioactive
metabolites. Its potential for therapeutic and medicinal applications is well established, both in modern and traditional
medicine. Research was focused towards the identification of the chemical composition for specimens of A. herba-alba,
but not the genetic diversity present within the same species. In this paper, replicate samples of the plant species were
collected from three distinct locations in both Mersa-Matrouh and Saint Catherine (Wadi El Raha). Genomic DNA was
extracted from aerial plant materials and amplified via SCoT-PCR to determine their genetic relatedness using Dice
coefficient to construct their dendrogram. SCoT-PCR associations indicated genetic variance within the species and close
association between (S2 & S3) and (M1 & M2).