One of the most important problems in the human health nutrition field is the use of food additives. Monosodium glutamate 
(MSG) as a flavor enhancer and aspartame (ASP) as a non-nutritive sweetener are two of the most widely used additives all 
over the world. The objective of the present study is to evaluate the effect of daily oral administration of MSG (13 mg/kg 
b.w.) and ASP (13 mg/kg b.w.) for one month either individually or in combination on different hematological parameters, 
lipid profile, liver and kidney functions of adult male albino rats. The data revealed that there was a significant reduction in 
platelets count and WBC count in both of MSG and ASP treated groups. While the combination of the two additives resulted 
in significant reduction in Hb, PCV, MCV, MCH and WBC values. There is a highly significant increase in HSP-70 levels in 
all treated groups when compared to the control group. The data revealed also that, both of ASP+MSG treated rats showed 
highly significant increase in lipid profile parameters (TG, TC, HDL, LDL, VLDL) and liver enzyme activities (ALT, AST, 
ALP). The levels of serum urea, creatinine and uric acid revealed a significant increase in all treated groups especially the
group of (ASP+MSG) treated rats. In conclusion administration of ASP and MSG to adult male albino rats caused 
disturbances in hematological parameters, lipid profile, liver and kidney functions of adult male albino rats. Getting more 
attention to the constituents of food products is more recommended to protect people from these additives.