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369757

Microbiological and molecular characterization of thermophilic Streptomycesvariabilis with antimicrobial activity

Article

Last updated: 29 Dec 2024

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Abstract

The rise of antibiotic resistance phenomena increased the interest for finding effective antimicrobial alternatives to control 
microbial pathogens. Actinomycetes represent a great reservoir for isolation and identification of antimicrobial compounds. 
In this study, various samples were randomly collected from the soil of different places in south Delta in Egypt. Two isolates 
of thermophilic Streptomyces spp were isolated on starch nitrate agar under thermophilic conditions, and were identified 
according to their morphological characteristics. Only, one isolate showed antifungal and antibacterial activity against 
various fungal and bacterial pathogens including Staphylococcus aureus, Escherichia coli and Streptococcus. Maximum 
antimicrobial activity was detected against Aspergillus niger. This isolate was identified as Streptomyces variabilisEG1on the 
base of the morphological characteristics and 16s rRNA gene sequencing. Optimization of some culture conditions was 
studied for attaining maximum antimicrobial agent production. This was achieved via adjusting carbon, nitrogen sources 
including starch and potassium nitrate, pH, time and temperature. Antimicrobial agent production by S.variabilis EG1 was 
observed on the 5th day of growth on the starch nitrate culture media (incubation at 50° C and pH 7.0) and was efficiently 
extracted by many solvents. This work confirmed the ability of thermophilic actinomycetes, S. variabilisEG1 to produce 
unpurified antimicrobial agents for pharmaceutical applications.

DOI

10.21608/jbes.2018.369757

Keywords

Thermophilic actinomycetes, Streptomyces, Antimicrobial, microbial pathogens

Authors

First Name

Ramy

Last Name

Fikry

MiddleName

-

Affiliation

Botany Department, Faculty of Science, Benha University, Benha, Egypt

Email

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City

-

Orcid

-

First Name

Ahmed G.

Last Name

Abdelhamid

MiddleName

-

Affiliation

Botany Department, Faculty of Science, Benha University, Benha, Egypt

Email

-

City

-

Orcid

-

First Name

Heba S.

Last Name

Essawy

MiddleName

-

Affiliation

Botany Department, Faculty of Science, Benha University, Benha, Egypt

Email

-

City

-

Orcid

-

First Name

Dina M.

Last Name

Baraka

MiddleName

-

Affiliation

Botany Department, Faculty of Science, Benha University, Benha, Egypt

Email

-

City

-

Orcid

-

First Name

Mahmoud M.

Last Name

Hazaa

MiddleName

-

Affiliation

Botany Department, Faculty of Science, Benha University, Benha, Egypt

Email

-

City

-

Orcid

-

Volume

5

Article Issue

1

Related Issue

49458

Issue Date

2018-01-01

Receive Date

2024-07-28

Publish Date

2018-01-01

Page Start

34

Page End

43

Print ISSN

2536-9202

Online ISSN

2356-6388

Link

https://jbes.journals.ekb.eg/article_369757.html

Detail API

https://jbes.journals.ekb.eg/service?article_code=369757

Order

369,757

Type

Original Article

Type Code

3,063

Publication Type

Journal

Publication Title

Journal of Basic and Environmental Sciences

Publication Link

https://jbes.journals.ekb.eg/

MainTitle

Microbiological and molecular characterization of thermophilic Streptomycesvariabilis with antimicrobial activity

Details

Type

Article

Created At

21 Dec 2024