Microbial fuel cells (MFCs) provide new opportunities for energy 
generation through conversion of organic matter to electricity by 
electricity-generating bacteria. The cell voltage and degradation rate 
of Glucose were monitored in a mediator less single chamber air 
cathode microbial fuel cell (ML-SCMFCs). Glucose was used as 
substrate by the MFC that was inoculated with aerobic sludge. 
Degradation metabolites in the anode solution of MFC were analyzed 
according to standard methods by closed reflux titrimetric Method. 
The morphology of the anode has been analyzed using Scanning 
Electron Microscopy. The results showed that electricity output 
increased with the Glucose concentration increased until at definite 
limit 5.0 g/l the current decreased because inhibition effect of glucose 
in relation to cyclic voltammetry, The MFC displayed a maximum 
power density of 52 mW/m2 at stable current density 275 mA/m2 and 
a maximum glucose degradation rate 94.4%. SEM show an intensive 
of bacterial biofilm communities on anodic electrodes which 
responsible for the electron shuttling to the electrode to generate 
electricity. These results demonstrate that Gulcose can be used for 
electricity generation in MFC for practical applications.