Transient Electromagnetic (TEM) data were acquired at 66 stations in El- Gallaba Plain, which lies at the western bank of the River Nile, to the west of Kom Ombo town in Upper Egypt, in order to characterize the subsurface resistivity structure corresponding to lithological layering succession and water bearing formation/s. TEM data were collected using TEM-Fast 48 system with acquisition software. Lithologic logs and well logging records of some existing water wells have been used to develop the initial model for TEM data inversion. The inversion of the acquired data revealed a lithological succession consists of six major layers at the center of the plain including a water bearing formation and other uplifted four older layers at the northeastern periphery of the plain. The level, resistivity and thickness of some selected layers were displayed in the form of contour maps, such as level contour map of the upper surface of the lowermost detected shale layer, water table map, iso-resistivity contour map and isopach contour map for the water bearing formation as well as a map for the inferred structural elements in the investigated area. Cross sections in E-W and N-S directions enabled recognizing the vertical and horizontal variations in the geoelectrical parameters of the detected layers (thicknesses and resistivities) and their correlation in addition to revealing the structural elements that affect the area in different directions. The water table map derived from the TEM interpretation revealed that the groundwater flow is mainly toward the central part of the plain, with a clear flow direction from south toward north. The alluvial deposits aquifer is likely recharged from the Nubian Aquifer in the south