Fish skin-derived collagen and Saccharomyces cerevisiae-derived beta-glucan were extracted for potential use in wound healing applications, with a comprehensive investigation into their physicochemical properties such as molecular weight, solubility, and heat stability. Fourier transform infrared spectroscopy (FTIR) was utilized to examine the chemical bonds of fish collagen and compare its resemblance to type 1 collagen. A mouse model was employed to assess the wound-healing impact of β-glucan. The findings revealed that employing a weak acid (CH₃COOH) in combination with a strong base (NaOH) for extraction resulted in high beta-glucan content and an advantageous ratio of polysaccharides, proteins, and lipids. Additionally, rats were utilized to evaluate the healing effects of the chosen β-glucan. The animal model demonstrated that the combination of collagen and β glucan exhibited superior efficacy in wound healing, leading to accelerated wound closure. Based on these results, the isolated fish collagen shows promise for potential utilization in wound treatment.