Fungi play a crucial role in the management of diseases in humans and other animals, making a highly significant contribution. Numerous biologically active compounds derived from fungi have been extensively studied, with these genera serving as sources for hundreds of different compounds. In a particular study, it was reported that the fungus Rhizopus stolonifer exhibited antimicrobial activity against selected fungal and bacterial pathogenic strains. This finding sparked interest in investigating other potential activities of this fungus. To conduct the study, Rhizopus stolonifer was obtained by incubating twenty hydrated bread at a temperature of 35 °C. The fungal material was then carefully collected and macerated in 70% ethanol for a period of approximately 7 days. The investigation focused on the anti-diabetic and antihypertensive activities of Rhizopus stolonifer, using a dose level of 25 mg/kg. The effects were assessed by measuring blood glucose levels with a digital glucometer and monitoring blood pressure with a non-invasive recorder apparatus before and after treatment. The comprehensive study revealed that the ethanolic extract of Rhizopus stolonifer significantly reduced blood glucose levels compared to the control group of mice with Alloxan-induced diabetes. However, there were no changes observed in systolic or diastolic blood pressure when compared to the control group of mice with fludrocortisone-induced hypertension. Furthermore, the histopathological examination demonstrated that Rhizopus stolonifer exhibited a protective and regenerative effect on beta cells in the pancreas of diabetic mice. Additionally, it displayed a protective effect on the renal tubules tissue in the kidneys of hypertensive mice.