Artocarpus heterophyllus Lam. is an average tree, commonly known as jackfruit, and belongs to the family Moraceae. Jackfruit was found native to central and eastern Africa. This work conducted on matured seedling trees growing under Aswan governorate condition on two Locations at both botanical Garden Island at Aswan and tropical farm at Kom Ombo Research Station and El Qalyobia Governorate at El-Kanater Horticulture Research station,  for three successive years during 2020 to 2022 seasons. The tree Genotype JF 7, produced the highest yield, fruits number and TTS Ratio (151 kg/plant, 82 fruit/plant and 22% respectively). In addition, it has the best branching characteristics as Semi Erect Considering the trees also could use as shade trees with Circumference 1.25m Semi Erect tree shape That is lead us to advises to multiply it and spread it in all the fields, allover of Egypt as an over loading load crop. And as micro climate modifying tree. Using RAPD analysis. The overall frequencies of polymorphic loci ranged from 324 to 2900 bp. Two loci were found at 900 bp in both of the genotype (JF-7 and JF-8) while absent in other genotypes. Two other loci.  At 400 and 1200were found at a similar frequency in (JF-9 and JF-10) and (JF-4 and JF-5) genotypes, respectively. Reveal that   genetic variation in Jackfruit could be used as a baseline study in estimation of genetic diversity in different Jackfruit genotypes in Egypt., this might allow breeders to develop improved varieties with new genetic combinations through selection.