Two seasons in a row (2023-2024) were consumed for this inquiry at El-Wahat El- Dakhla in the governorate of the New Valley. There were two sections of the current study. The first goal was to choose date palm genotype trees from the specified site that had high-quality fruit. Six semi-dry genotypes date palms were examined for their fruit's physical and chemical characteristics in comparison to the Sultany variety. Date palm genotype No. 3 (97.66 units), No. 1 (91.27 units), No. 4 (86.78 units), No. 6 (84.4 units), No. 2 (82.68 units), No. 5 (81.46 units), and Sultany cultivar (63.56 units) were the results of the numerical selection of the chosen palms. The second objective of this study is to use inter-simple sequence repeats (ISSR) as a DNA fingerprint to identify the genetic diversity among chosen genotypes.