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333505

Suppression and Molecular identification of severe Cercospora beticola causing Cercospora leaf spot disease on sugar beet

Article

Last updated: 29 Dec 2024

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Tags

Plant Pathology

Abstract

Cercospora leaf spot disease, triggered by Cercospora beticola, poses a substantial threat to sugar beet varieties. Evaluation of nineteen sugar beet varieties indicated that Perfekta, Smart meyra KWS, Aseel, and Vulkhan exhibited the lowest disease severity and incidence. At the same time, Polylias, BTS 3880, Prilive, Faralda Kws, Zoom, and Smart Seza KWS showed higher susceptibility. The causal organism was isolated from diseased sugar beet plants in Kafrelshiekh, Behira, and Dakahlia governorates during the 2020 growing season. Twenty-seven fungal isolates were obtained and evaluated for aggressiveness on susceptible sugar beet variety (Polylias cv). Phylogenetic analysis using ITS sequences revealed genetic variations among the four most aggressive isolates, which were subsequently registered in NCBI. Furthermore, the efficacy of essential oils Citronella and Rose Geranium (Pelargonium graveolens) in controlling C. beticola was examined. Pelargonium graveolens at 10 ml/L demonstrated the highest effectiveness in reducing fungal growth, as well as in increasing total soluble solids content and sucrose. Additionally, this essential oil enhanced the activity of polyphenol oxidase and peroxidase enzymes. Scanning electron microscopy (SEM) examination confirmed that Citronella and Pelargonium graveolens, along with the fungicide montoro 30%, induced plasmolysis, decomposition, and damage to conidiophores and conidiospores of C. beticola, contrasted to the control treatment. This integrated approach, combining molecular characterization, disease evaluation, and essential oil application, provides valuable insights for the management of Cercospora leaf spot on sugar beet, offering sustainable and environmentally friendly alternatives to traditional fungicides.

DOI

10.21608/jpfs.2023.259788.1006

Keywords

Cercospora beticola, Pelargonium graveolens, Cymbopogon citratus, Citronella, Rose geranium, ITS sequence

Authors

First Name

Mohamed

Last Name

Sehsah

MiddleName

D.

Affiliation

Plant Pathology Research Institute, Agricultural Research Center, Giza 12619, Egypt

Email

-

City

-

Orcid

-

First Name

Reda

Last Name

Omara

MiddleName

Ibrahim

Affiliation

Plant Pathology Research Institute, Agricultural Research Center, 12619, Giza, Egypt.

Email

redaomara43@arc.sci.eg

City

-

Orcid

0000-0002-9776-0238

First Name

Samar

Last Name

Elsayed

MiddleName

S.A.

Affiliation

Plant Pathology Research Institute, Agricultural Research Center, Giza 12619, Egypt

Email

-

City

-

Orcid

-

First Name

Walaa

Last Name

Abdelghany

MiddleName

R.

Affiliation

Plant Pathology Research Institute, Agricultural Research Center, Giza 12619, Egypt

Email

-

City

-

Orcid

-

First Name

Heba

Last Name

Saad El-Din

MiddleName

I.

Affiliation

Plant Pathology Research Institute, Agricultural Research Center, Giza 12619, Egypt

Email

-

City

-

Orcid

-

First Name

Heba

Last Name

Afifi

MiddleName

-

Affiliation

Gene Bank, Agriculture Research Center, Giza, Egypt.

Email

-

City

-

Orcid

-

Volume

1

Article Issue

2

Related Issue

45286

Issue Date

2023-12-01

Receive Date

2023-11-01

Publish Date

2023-12-01

Page Start

56

Page End

72

Print ISSN

3009-7487

Online ISSN

3009-7886

Link

https://jpfs.journals.ekb.eg/article_333505.html

Detail API

https://jpfs.journals.ekb.eg/service?article_code=333505

Order

333,505

Type

Original Article

Type Code

2,955

Publication Type

Journal

Publication Title

Journal of Plant and Food Sciences

Publication Link

https://jpfs.journals.ekb.eg/

MainTitle

Suppression and Molecular identification of severe Cercospora beticola causing Cercospora leaf spot disease on sugar beet

Details

Type

Article

Created At

20 Dec 2024