New biotechnologies that would enable a decrease in the usage of chemical inputs without adversely affecting crop output or farmers' income are being sought after by modern agriculture. Vermicompost and seaweed extract are used as nutrient supplements, organic fertilizers and biostimulants in agriculture and horticulture to increase plant growth and yield. This study was conducted at private farm in Beni-Suef, during the two successive years of 2020/2021 and 2021/2022 to investigate the effects of vermicompost (VC), seaweed extract (SW), and chemical fertilizers on the growth, physiological and yield characteristics of evening primrose. The obtained results show that the combination treatment of vermicompost at 2 tons/fed and seaweed extract at 400 ppm gave the highest values of growth characteristics, seed yield and linoleic acid content of oil.