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338217

Double Ionophore Activation (DIA) technique in cases of severe male factor: Case Series

Article

Last updated: 29 Dec 2024

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Subjects

-

Tags

Embryology

Abstract

Background: Sperm binding or injection into human oocytes induces calcium oscillations resulting in oocyte activation, fertilization, and subsequent embryonic development.  In severe male factor, sperm may fail to activate oocytes with subsequent ICSI failure.  Artificial oocyte activation with calcium ionophore can induce calcium influx with improved oocyte activation and development.
Objective: The current case series evaluated a unique technique of double ionophore activation (DIA) in severe male factor without previous ICSI failure.
Materials and Methods: Case series of 17 infertile couples with severe male factors undergoing ICSI. The injected oocytes were incubated in calcium ionophore twice with this new protocol for artificial oocyte activation.
Results: Fertilization, cleavage, and blastocyst rates were 71.4%, 100%, and 73.3% respectively.  The pregnancy rate for fresh transfer was 9/13 (69.2%) and the pregnancy rate for FET was 4/6 (66.7%).
Conclusion: DIA protocol may improve oocyte activation in severe male factor and a well-designed prospective study is needed.

DOI

10.21608/jrme.2024.264068.1007

Keywords

oocyte, oligoathenoteratozoospermia, fertilization, ICSI, Blastocyst rate

Authors

First Name

Dalal

Last Name

Guiziry

MiddleName

-

Affiliation

Clinical Pathology Department, Faculty of Faculty of Medicine Alexandria University, Egypt., Dar Alteb Infertility Center, Alexandria, Egypt., Egyptian Foundation of Reproductive Medicine and Embryology (EFRE), Egypt.

Email

efre.publications@gmail.com

City

-

Orcid

-

First Name

Nehal

Last Name

Adel

MiddleName

-

Affiliation

Egyptian Foundation of Reproductive Medicine and Embryology (EFRE), Egypt., Madina ICSI Fertility Center, Madina women's hospital, Alexandria, Egypt.

Email

nehal_adel83@hotmail.com

City

-

Orcid

0000-0002-2853-8334

First Name

Mohammed

Last Name

Faris

MiddleName

-

Affiliation

Dar Alteb Infertility Center, Alexandria, Egypt., Obstetrics and Gynecology Department, Faculty of Medicine, Ain Shams University, Egypt

Email

mohammed.ahmed@med.asu.edu.eg

City

-

Orcid

-

First Name

Esraa

Last Name

Fathy

MiddleName

-

Affiliation

Dar Alteb Infertility Center, Alexandria, Egypt.

Email

esraafathy1266@gmail.com

City

-

Orcid

-

First Name

Ashraf

Last Name

Abo Ali

MiddleName

-

Affiliation

Egyptian Foundation of Reproductive Medicine and Embryology (EFRE), Egypt., Madina ICSI Fertility Center, Madina women's hospital, Alexandria, Egypt.

Email

aboalimadinahospital@gmail.com

City

-

Orcid

-

First Name

Heba

Last Name

Maghraby

MiddleName

-

Affiliation

Dar Alteb Infertility Center, Alexandria, Egypt., Egyptian Foundation of Reproductive Medicine and Embryology (EFRE), Egypt.

Email

hebahali85@gmail.com

City

-

Orcid

-

First Name

Mohamed

Last Name

Elmahdy

MiddleName

-

Affiliation

Egyptian Foundation of Reproductive Medicine and Embryology (EFRE), Egypt. , Obstetrics and Gynecology Department, Faculty of Medicine, Alexandria University, Egypt.

Email

mahdy_moh@yahoo.com

City

-

Orcid

0000-0003-4332-9845

First Name

Hassan

Last Name

Maghraby*

MiddleName

-

Affiliation

Dar Alteb Infertility Center, Alexandria, Egypt., Egyptian Foundation of Reproductive Medicine and Embryology (EFRE), Egypt., Obstetrics and Gynecology Department, Faculty of Medicine, Alexandria University, Egypt.

Email

hassanmaghraby@gmail.com

City

-

Orcid

-

Volume

1

Article Issue

1

Related Issue

45864

Issue Date

2024-01-01

Receive Date

2024-01-20

Publish Date

2024-01-01

Page Start

60

Page End

65

Online ISSN

3009-7282

Article File

JRME_Volume 1_Issue 1_Pages 60-65.pdf

PDF . 286.6kB

Link

https://jrme.journals.ekb.eg/article_338217.html

Detail API

https://jrme.journals.ekb.eg/service?article_code=338217

Order

9

Type

Case Series

Type Code

3,043

Publication Type

Journal

Publication Title

Journal of Reproductive Medicine and Embryology

Publication Link

https://jrme.journals.ekb.eg/

MainTitle

Double Ionophore Activation (DIA) technique in cases of severe male factor: Case Series

Details

Type

Article

Created At

20 Dec 2024