Two factorial experiments in split plot design were carried out during the two successive growing seasons of 2018/2019 and 2019/2020 at Soils, Water, and Environ. Res. Inst. Farm in Sahl El-Tina Agric. Res. Station, Port Saied Governorate, Agric., Res. Center, Egypt to investigate the effects of some plant growth promoters (PGPs) (Azospirillum lipoferum, Azotobacter chroococcum, Bacillus subtilis, and Bacillus polymyxa) alone or with different organic substances (compost (Com) at 4 ton/fed, ascorbic acid (AsA), compound amino acids (CAA) at 200 ppm for each) and their interactions on fennel (Foeniculum vulgare Mill.) growth and soil chemical properties under saline-sodic soil conditions. Significant increases in plant height (cm), number of branches and umbels/plant, fresh and dry weights/plant (g), weight of 100 fruits (g), fruit yield/plant (g), essential oil % and yield/plant (ml) were recorded. The interaction between all organic substances and PGPs treatments decreased the estragole content values. The percentage of total carbohydrates, N, P, and K in fruits as well as soil dehydrogenase and nitrogenase activities increased with the tested treatments, especially Com + AsA + CAA with (PGPs). Proline content in fresh leaves decreased, and soil pH and EC were enhanced especially in the case of organic substances with PGPs treatments which reflected in fennel quantity and quality. The application of organic substances (Com at 4 ton/fed, AsA and CAA at 200 ppm for each) with PGPs (A. chroococcum, A. lipoferum, B. polymyxa, B. subtilis) could be recommended for enhancing fennel yield and essential oil under saline-sodic soil conditions.