The study was conducted to evaluate Alchemilla vulgaris extract in reducing the symptoms of inflammation and ulceration in rats with ulcerative colitis. The study conducted on 25 rats, which was divided into five groups each group five rats. Group (1( negative control, Group)2( positive control, Group (3)(drug) it was given 1 cm of sulfazine, Group)4) it was given 150mg from Alchemilla vulgaris herb extract and Group)5) it was given 200mg from Alchemilla vulgaris herb extract. Groups )2, 3, 4, and 5( were injected with 2 cm of acetic acid at a concentration of 4% into the rectum and left for three days to confirm the occurrence of the disease. The treatment by Alchemilla vulgaris herb extract and sulfazine was daily for a month. The results showed in blood samples decrease in the average value of (WBCs, ESR, MCV, Neutrophil) while the average values of (RBCs, Hct, HB, MCH, Lymph) increased ,in(3, 4, and 5 groups (. When comparing group (2) with groups (3 and 5) in some hematological parameters was P.value (P ≤0.01). When comparing group (2) with group (4) in some hematological parameters was P.value (P ≤0.05). Our results showed that the treated group with 150mg , 200mg Alchemilla vulgaris and drug (sulfazine) showed improvement in some hematological parameters.