Cancer is the second killer disease of the world, and its management is a major socio-medical problem. Although, several chemotherapeutic approaches have been taken to tackle the complexity of different cancer, toxicity and resistance may represent the potential obstacles to successful clinical development. Search for novel and safe drug from natural plant products are ventured worldwide. This study aims to show the impact of Parotoid gland secretions (PGS) of the Egyptian toad (Bufo regularis) on the kidney functions of Ehrlich ascites carcinoma bearing mice (EAC). PGS were collected to evaluate their in vivo anti-tumor effect, the median lethal dose (LD50) of PGS was determined. Forty female Albino mice were separated into four groups. Group 1 considered the control. Groups 2 to 4 were injected 1× 106 EAC intraperitoneally (ip). Group 2 considered the positive control. Groups 3 received ip injection of cisplatin (Cis) (2 mg/kg), while Group 4 injected with PGS 1/10 (78 mg/kg). PGS and Cis were ip injected daily for 7 days. After 14 days of treatment, blood samples were collected, the b.wt change, tumor indices and biochemical parameters were investigated. PGS effects used for EAC treatment are carried out by kidney function. Serum urea and creatinine were evaluated to reflect any clinical changes as well as histological alterations of renal tissues were investigated. The kidney functions were improved in EAC-bearing mice treated with PGS, as evidenced by ameliorating their histological structure. The study showed that PGS has a moderate antitumor effect on EAC- bearing mice.