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316733

Molecular characterization of plantacirin C produced from Lactobacillus plantarum isolated from dairy products sold in Assuit city, Egypt

Article

Last updated: 05 Jan 2025

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Abstract

Last decade, bacteriocins have most concern due to their benefits in human health and immunity. One of the most important bacteriocin-producing bacteria is Lactobacillus plantarum (L. plantarum). Thus, this study aimed to screen for plantacirin C (plnc) predominant bacteriocin produced from L. plantarum in traditional dairy products (Milk, Cheese and yoghurt) of Assuit city in Upper Egypt. Molecular method has been used for the detection of bacteriocin-encoding genes (plnc) produced after isolation L. plantarum and identified by biochemical methods.
The findings showed that, at a proportion of 54.3%, 51 strains of L. plantarum were isolated from raw milk and conventional dairy products. Polymerase chain reaction (PCR) was used to find the genes encoding bacteriocin. Based on the molecular technique, 6 out of 51 isolates were carried plnc gene. The results concluded that raw milk is the highest sample containing plnc gene. More studies for molecular identification of plantacirin c in Egyptian dairy products should be advised. Bacteriocin has a lot of benefits for animal and human health so should be used as a natural bio-preservative, medicinal and a natural substitute for antibiotics.

DOI

10.21608/jamb.2023.217829.1005

Keywords

Bacteriocin gene, Raw milk, cheese, Lactic acid bacteria (LAB)

Authors

First Name

Eman

Last Name

Gad

MiddleName

-

Affiliation

Molecular Biology Research& Studies Institute, Assiut University, Egypt.

Email

emyfarouk791@gmail.com

City

-

Orcid

-

First Name

Walaa

Last Name

Elsherif

MiddleName

Mahmoud

Affiliation

Department of Food Hygiene and Nano-technological Research and Synthesis Unit at Animal Health Research Institute, Agricultural Research Center, Assiut university ,Egypt

Email

me.elsherif@yahoo.com

City

-

Orcid

-

First Name

Rania

Last Name

Ewida

MiddleName

Mohammed

Affiliation

Department of Food Hygiene, Faculty of Veterinary Medicine, New Valley University.Egypt

Email

r_ewida@yahoo.com

City

Assiut

Orcid

-

First Name

Bahaa

Last Name

Abdel-Fattah

MiddleName

-

Affiliation

Department of Genetics, Faculty of Agriculture, Assiut University, Egypt.

Email

elhayq@yahoo.com

City

-

Orcid

-

Volume

1

Article Issue

1

Related Issue

43423

Issue Date

2023-09-01

Receive Date

2023-06-15

Publish Date

2023-09-01

Page Start

83

Page End

92

Online ISSN

2974-4008

Link

https://jamb.journals.ekb.eg/article_316733.html

Detail API

https://jamb.journals.ekb.eg/service?article_code=316733

Order

316,733

Type

Original Article

Type Code

2,814

Publication Type

Journal

Publication Title

Journal of Applied Molecular Biology

Publication Link

https://jamb.journals.ekb.eg/

MainTitle

Molecular characterization of plantacirin C produced from Lactobacillus plantarum isolated from dairy products sold in Assuit city, Egypt

Details

Type

Article

Created At

20 Dec 2024