The present study was conducted during 2021 in the Forestry and Timber Trees Dept., Hort. Res. Inst., A R C, Giza, Egypt. The study aimed to evaluate biomass and some wood properties of second rotation of Eucalyptus gomphocephala Dehn at age of 22- years- old. And selecting the possibilities of using of E. gomphocephala wood. Mean values of total height, merchantable height, DBH (diameter at breast height), basal area and volume per tree were 19.72 ± 1.42 m, 16.51 ± 0.32 m, 118.49 ± 15.23 cm, 0.40 ± 0.08 m2 and 3.49 ± 0.48 m3, respectively. Mean green and dry weight of stem, and branches were (3.54 ± 0.57 and 2.56 ± 0.41), and (0.69 ± 0.18 and 0.60 ± 0.16) ton / tree, respectively. Mean values of wood density were reduced with stem height up. The highest values of strength property of wood as a modulus of rupture (MOR), and compressive strength parallel to grain (C max) were at level 2nd (DBH +2.5 m). The highest values of hardness property as Janka hardness number (JHN) in radial and tangential directions were at 1st level (DBH). At 2nd level, cellulose and lignin were highest values, and reduced with stem height up.