This experiment was carried out in Plant Tissue Culture Laboratory at El-Zohryia Botanical Garden, Hort. Res. Instit., Agric. Res. Center, Egypt during the period of 2022 to 2023.The objective of this study was to assess a protocol for micro-propagation of Yucca desmetiana plant. Terminal buds were sterilized with Clorox at (20, 25 or 30 %) or mercuric chloride (MC) solution (Hgcl₂) at (0.1, 0.2 or 0.3 %) for 15, 20 and 25 min for sterilizing explants. In establishment stage, various MS medium strengths (¼, ½, ¾ or full strength) were used. For multiplication stage various concentrations of BAP (0, 1, 3, 5, 7, 9 or 11 mg/l) were examined. At rooting stage, IBA or NAA at different concentrations (0, 1, 3, 5 or 7 mg/l) were tested. Also, different media types were noticed during acclimatization stage. The obtained results referred to that sterilizing explants with Clorox 25 % for 25 min gave the best result of survival percentage (100 %), while the lowest contamination percentage were noticed when explant was exposed to MC at (0.1, 0.2 and 0.3 % for 15 min). The highest shoots  in establishment stage were achieved with ¾ or full strength of MS medium. Moreover, 5 mg/l BAP gave the highest values in multiplication stage for the same parameters. Using NAA at 7 mg/l concentrations recorded the longest roots during rooting stage while IBA at 7 mg/l gave more roots. Media mixture of peat moss+ sand +perlite (1: 1: 1 v/v/v) at acclimatization stage.