Two experiments were executed during the seasons of 2022 and 2023 at a commercial nursery. The first experiment was done to assess the efficiency of willow water extract (WWE) and indole-3-butyric acid (IBA) on the rooting of Petunia x hybrida stem cuttings, seven treatments were done in this experiment (control, 500 ppm IBA, 1000 ppm IBA, 2000 ppm IBA, 25% WWE, 50% WWE and 100% WWE). The results showed that appliction of IBA at 500 ppm resulted in the highest survival percentage and the highest increase in root characteristics. The second experiment was carried out to study the effects of IBA, WWE with or without micronutrient fertilizer (MF) supplementation. MF consists of zinc (Zn) and iron (Fe) at 13 % was prepared at 0.5 g/l. and sprayed on the leaves of the stem cuttings three times , the first time one week from planting, and then at a week intervals. Twelve treatments were done in this experiment (control, 25 % WWE, 50% WWE, 125 ppm IBA. 250 ppm IBA, 500 ppm IBA, Control + MF, 25% WWE + MF, 50% WWE + MF, 125 ppm IBA + MF , 250 ppm IBA + MF and 500 ppm IBA + MF). The results showed that the highest survival percentage, root parameters, vegetative growth characteristics and chlorophyll content was obtained after application of 125 ppm IBA + MF. Also, the profit percentage of using 125 ppm IBA + MF to produce petunia plants by stem cuttings was assessed and recorded 100.98 %.