This study was carried out at a private farm at Wardan, Giza Governorate, Egypt, during the two seasons of 2018/2019 and 2019/2020. The aim of this study was to investigate the effect of nursery mother plants removal dates (after 3, 4, 5 months) from planting the nursery in addition to control (without mother plants removal) on transplants quality, production and fungal diseases severity in roots and crown of six strawberry cultivars "Festival, Fortuna, Florida, Eliana, Sensation and Monterey". The experimental design was a split plot design with three replications. The soil type of experimental site was sandy with pH of 6.77 and EC of 0.6Mm. The results indicated that there were significant differences among the tested strawberry cultivars in vegetative growth characteristics (plant length, crown diameter and root length) of their transplant. Festival cultivar gave the highest transplants number while Florida variety seedlings have the highest carbohydrate content. Removing mother plant increased crown diameter of the produced transplants and number of transplants. Delaying mother removal date increased significantly the disease incidence values. Moreover, control treatment (without removal) showed significantly the highest value as compared with the tested treatments. Earlier removing mother transplant after three months for Festival cultivar give the highest number of transplants. The study recommend removing mothers plants of the strawberry nursery after three months from planting of nursery to improve number of transplants, quality and decrease the infection and the severity of the fungi attacking roots and crown which cause root and crown rot diseases.