This study was conducted to assess greenhouse cucumber productivity and fruit chemical composition from the application of chicken manure and compost. Cucumber seedlings "cv. Barracuda F1" were transplanted in autumn and spring cultivation of 2021-2023 in a 8.5 x 40 m single arch plastic greenhouse at Bosaily. Each of the three beds, apart from the two side beds, was divided into three sections of about 12m, representing one replicate of each of the three treatments. Each replicate had 48 plants in two rows. The treatments were: farm waste compost, chicken manure, and the control. Compost and chicken manure were added to the bottom of the bed at a rate of 50 kg per replicate. The results showed that the total yield ranged from 1.9 to 3.3 Kg per plant. The highest early yield was obtained under chicken manure, followed by compost (1.9 and 1.8 kg/plant respectively). The application of chicken manure or compost improved cucumber productivity in the greenhouse by up to 11.5%. The effect of modifying organic matter accumulation on microelements and heavy metals in cucumber fruits was not clear as this may require further studies over a longer time. The chemical analysis of fruits showed that only Fe, Mn, and Pb were lower than the permissible limits of WHO/FAO concentration in agricultural soil. In contrast, Zn, Cu, and Cd concentrations were higher in organically amended soil. Ni concentration was higher than the limit in all treatments and all seasons. These results indicate the necessity of monitoring heavy metals in the soil, especially after successive vegetable cultivation under greenhouses.