The aim of this study was to reach a well-defined protocol to in vitro propagate of Arganiaspinosaand overcome vitrification phenomenon. In this respect, the highest number of shoot was found at 2.0 mg/l Kin at genotype 3 for multiplication stage. MS medium supplemented with 100 mg/l calcium pantothenate was suited for to eliminate vitrification at all genotypes. For callus induction, MS medium supplemented with 1.5 mg/l NAA when incubated in the light or darkness was favorable to callus induction. MS medium supplemented with 0.5 mg/l GA₃ + 1.5 mg/l BA led to increases shoot initiation.