This study was carried out during (2020/2021) seasons on softwood cuttings of mature trees of guava cv. Montakab El-Sabahia conducted at the El-Sabahia Experimental Horticulture Research Institute in Alexandria Governorate, Egypt. The softwood cuttings were prepared on two separate dates: the last week of April and July. The basal ends of the cuttings (3–4 cm) were dipped for 5 minutes in solutions of Indole-3-butyric acid (IBA) at 2000 and 3000 ppm, NAA at 500 and 1000 ppm, and bio solution before planting each year. The more favorable results were obtained by the cuttings t that collected on the first date (last week of April). Among the treatments, treating cuttings by 3000 ppm IBA achieved high results in terms of percentage of rooted cuttings (30.7%), number of roots per cutting (5.5), root fresh weight per cutting (4.5 g), and survival percentage of rooted cuttings (47.3%). However, the highest length of roots (7.1 cm) was observed at 1000 ppm NAA treatment.