Water and soil salinity were the biggest obstacles to achieving the world's supply - demand ratio, so with the growing population there is no choice but to use salty soil and water in agriculture, and the need for environmentally friendly technologies has emerged to overcome the adverse effects. Therefore, the effect of the halotolerant plant growth-promoting rhizobacteria (HT-PGPR) with organic fertilizers and spirulina algae on the biochemical characters as chlorophyll content, NPK %, total carbohydrates, phenols, proline and antioxidant enzymes activities of pomegranate transplants (Wonderful cv.) under different irrigation water salinity levels (6000, 7000 and 8000 ppm) were investigated. Pot experiment was conducted in Horticulture Research Institute (HRI) nursery, Agriculture research center (ARC), Giza governorate, Egypt, under the greenhouse conditions using compost, halotolerant PGPR (Azotobacter chrococcum, Azospirillum lipoferum, Bacillus megaterim var. phosphaticum and Bacillus circulance) in addition to, Spirulina platensis L. algae in 2019 and 2020. Results indicated that, the organic and biofertilizers reduced soil pH and EC and increased the soil enzymatic activity under saline water condition. The leaf contents of total chlorophyll, carbohydrates, phenols, proline of transplants were increased, in addition to a significant increase in the nutritional elements NPK % in dry leaves were recorded when transplants were treated using PGPR inoculations, excluding sodium and chlorine. The highest efficiency in alleviating irrigation water salinity stress on pomegranate transplants was obtained from treatment contents compost + biofertilizers + spirulina algae with 6000 ppm salinity level compared to the chemical fertilizer treatment. The results of the study indicated that organic fertilizers with halotolerant PGPR inoculations can alleviate the deleterious effects of salt stress conditions and improve the tolerance and healthy growth of pomegranate transplant under salt water irrigation.