The purpose of this research is to develop a protocol for micro propagation on Spathiphyllum wallisii Regel. This study was carried out during the years 2020 and 2021. In the sterilization phase using 5% clorox for 15 min recorded very good results for survival and free contamination %. In establishment, full salt strength MS medium + 30 g/l sucrose increased shootlet formation. Chlorophyll content increased on full salt strength MS medium with 20 g/l sucrose while augmented with 40 g/l increased chlorophyll b. On the other hand, ¼ salt strength MS with 30 g/l sucrose increased carotenoids. In multiplication, adding 1.5 mg/l BAP resulted in the largest number of shootlets, while using 0.5 mg/l BAP recorded the largest number of leaves. Adding 0.5 mg/l Kin increased the contents of chlorophyll a and b while carotenoids increased with BAP at 1.5 mg/l. For rooting, culture on ¼ strength medium without or with activated charcoal gave the highest root numbers. There were no significant differences in root length. At acclimatization, studying the effect of media substrate (peat moss, peat moss + perlite or peat moss + sand) recorded good results without significant effect on the length, leaf number, root length and root number of plantlet.