This investigation was conducted on 10- year- old Aggizi olive trees grown in a private orchard located at Qena Valley, Qena Governorate aiming to study the effect of foliar applications of chitosan and amino acids on growth, leaf mineral composition, yield and fruit quality during the 2019 and 2020 seasons. The beneficial effects of spraying chitosan and amino acids on growth and productivity of Aggizi olive trees under winter warm climate and hot summer in Qena Governorate, were investigated. This experiment was designed and arranged in a complete randomized block design with four replicates per treatment, one Aggizi olive tree per each. The results indicated that all treatments were effective on enhancing vegetative growth, leaf minerals content and improving the yield and fruit quality of Aggizi olive trees comparing with checked treatment. The superior treatment regarding the improvement of vegetative growth, leaf nitrogen, phosphorus and potassium contents, flowering traits, yield (Kg/tree) and fruit quality were obtained with spraying chitosan 1 % + amino acids 2 % in both seasons. It can be concluded that, foliar application of chitosan 1 % + amino acids 2%  is useful in the improvement of vegetative growth, nutritional status of Aggizi olive trees and produced a high yield with good fruit quality under Qena Governorate conditions.