In this study, the effects of two rootstocks (Nemagard and Maryana) on the African Rose plum cv. degree of self and cross-compatibility with Hollywood and Pioneer cultivars, as well as, the leaf mineral content (calcium, boron, and zinc) were examined. Fluorescent microscopy studies postulated that self-pollinated flowers on two studied rootstocks showed self-compatibility. Nevertheless, a high degree of self-compatibility was observed on ‘Maryana' rootstock. Moreover, cross-compatibility was noted in styles crossed by ‘Hollywood' pollens and high degree of cross-compatibility was observed on ‘Maryana' rootstocks. Partially cross-compatible was observed in styles crossed by ‘pioneer' pollen, and high level of partial cross compatibility was observed on ‘Maryana'. While a lower degree was noted on ‘Nemagard'. Leaf analysis showed that ‘Maryana' had significantly higher calcium, boron and zinc for African Rose plum cv. mineral element concentration than ‘Nemagard'. Field data confirmed fluorescent microscopy results, rootstock with the higher fruit set percentage was ‘Maryana' when ‘African rose' flowers crossed by ‘Hollywood' pollens as well as self-pollinated flowers in comparison with ‘Nemagard' rootstock. In addition, ‘Maryana' was found to have significantly higher fruiting percentage than ‘Nemagard' when used ‘Pioneer' as a male parent. The data revealed that rootstock influences depend on increased nutrient absorption, which results in more fertilized flowers.